Influenza A Virus M 2 Ion Channel Activity Is Essential for Efficient Replication in Tissue Culture

Abstract

ABSTRACT The amantadine-sensitive ion channel activity of influenza A virus M 2 protein was discovered through understanding the two steps in the virus life cycle that are inhibited by the antiviral drug amantadine: virus uncoating in endosomes and M 2 protein-mediated equilibration of the intralumenal pH of the trans Golgi network. Recently it was reported that influenza virus can undergo multiple cycles of replication without M 2 ion channel activity (T. Watanabe, S. Watanabe, H. Ito, H. Kida, and Y. Kawaoka, J. Virol. 75:5656–5662, 2001). An M 2 protein containing a deletion in the transmembrane (TM) domain (M 2 -del 29–31 ) has no detectable ion channel activity, yet a mutant virus was obtained containing this deletion. Watanabe and colleagues reported that the M 2 -del 29–31 virus replicated as efficiently as wild-type (wt) virus. We have investigated the effect of amantadine on the growth of four influenza viruses: A/WSN/33; N 31 S-M 2 WSN, a mutant in which an asparagine residue at position 31 in the M 2 TM domain was replaced with a serine residue; MUd/WSN, which possesses seven RNA segments from WSN plus the RNA segment 7 derived from A/Udorn/72; and A/Udorn/72. N 31 S-M 2 WSN was amantadine sensitive, whereas A/WSN/33 was amantadine resistant, indicating that the M 2 residue N 31 is the sole determinant of resistance of A/WSN/33 to amantadine. The growth of influenza viruses inhibited by amantadine was compared to the growth of an M 2 -del 29–31 virus. We found that the M 2 -del 29–31 virus was debilitated in growth to an extent similar to that of influenza virus grown in the presence of amantadine. Furthermore, in a test of biological fitness, it was found that wt virus almost completely outgrew M 2 -del 29–31 virus in 4 days after cocultivation of a 100:1 ratio of M 2 -del 29–31 virus to wt virus, respectively. We conclude that the M 2 ion channel protein, which is conserved in all known strains of influenza virus, evolved its function because it contributes to the efficient replication of the virus in a single cycle.