Affiliation:
1. Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
Abstract
A rabbit homologous polyclonal antiserum to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was specifically produced and used in an antigen capture enzyme-linked immunosorbent assay (ELISA) to detect swine serum antibodies to this potentially important virulence factor. Sera from pigs experimentally infected with the most common disease-producing serotypes (serotypes 1, 2, 5, and 7) of A. pleuropneumoniae produced positive results in this ELISA. Of 144 serum samples collected from 10 herds free of pleuropneumonia and 155 serum samples from 11 herds with a history of the disease, 68 (47%) and 148 (95%), respectively, were found positive by the ELISA. In addition, pigs naturally infected with Actinobacillus suis produced antibodies which seroreacted in this ELISA. The results indicated that a high proportion of swine have antibodies seroreactive with the 104-kilodalton hemolysin produced by A. pleuropneumoniae.
Publisher
American Society for Microbiology
Reference22 articles.
1. Devenish J. and S. Rosendal. 1989. Identification of the heatlabile hemolysin of Actinobacillus pleuropneumoniae serotype
2. Can. J. Vet. Res. 53:251-254.
3. Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli;Devenish J.;Infect. Immun.,1989
4. Isolation and biological characterization of 2 lipopolysaccharides and a capsular-enriched polysaccharide preparation from Haemophilus pleuropneumoniae;Fenwick B. W.;Am. J. Vet. Res.,1986
5. Regulation of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca 2;Frey J.;Infect. Immun.,1988
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