In Vitro Attachment of Radioactive Endotoxins to Lysosomes-Reference-Cited by-同舟云学术

In Vitro Attachment of Radioactive Endotoxins to Lysosomes

Published:1971-11 Issue:5 Volume:4 Page:532-536
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Bona C.¹,Chedid L.¹,Lamensans A.¹

Affiliation:

1. Centre de Physiologie et d'Immunologie Cellulaire, Hôpital St. Antoine, Centre National de la Recherche Scientifique, and Service de Chimiothérapie Expérimentale, Institut Pasteur, Paris, France

Abstract

The experiments reported here demonstrate that under certain conditions endotoxin can interact with lysosomes in vitro. After incubation of large granular fraction with 51 Cr-labeled antigen under the conditions required for acid hydrolytic activity, radioactivity was associated with the pellet after centrifugation. This effect can be inhibited by preincubation of the large granular fraction with unlabeled homologous or heterologous endotoxins. High resolution autoradiography showed that 14 C-labeled endotoxin was predominantly attached to the lysosomes contained in the large granular fraction. The mechanism of this interaction and its relationship to the toxic effect of endotoxins on lysosomes are discussed.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.4.5.532-536.1971

Reference24 articles.

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