Affiliation:
1. Department of Biochemistry, University of Tsukuba, Ibaraki, Japan.
Abstract
To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fetuses. Moreover, 71 offspring were delivered by cesarean section from the remaining seven dams and fostered by seven normal rats. HTLV-I provirus was detected in peripheral blood mononuclear cells in 2 of the 71 offspring 4 weeks after cesarean section. These results indicate for the first time the intrauterine transmission of HTLV-I. To confirm the postnatal transmission, MT-2 cells were injected into a dam within 24 h after delivery, and six offspring were fostered by this dam. HTLV-I provirus was detected in peripheral blood mononuclear cells of all six offspring. This animal model may be useful for analysis and prevention of mother-to-child transmission of HTLV-I.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference35 articles.
1. Optimal conditions for hybridization with oligonucleotides: a study with myc-oncogene DNA probes;Albretsen C.;Anal. Biochem.,1988
2. Imaging plate illuminates many fields;Amemiya Y.;Nature (London),1988
3. Vertical transmission of HTLV-1 in HTLV-1 carrier rat;Ami Y.;Jpn. J. Cancer Res.,1992
4. Transmission of adult T-cell leukemia retrovirus (HTLV-I) from mother to child: comparison of bottle- with breast-fed babies;Ando Y.;Jpn. J. Cancer Res. (Gann),1987
5. Beaudoin A. R. 1988. Embryology and teratology p. 76-101. In H. J. Baker J. R. Lindsey and S. H. Weisbroth (ed.) The laboratory rat vol. II. Academic Press New York.
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