Identification of BV/ODV-C42, an Autographa californica Nucleopolyhedrovirus orf101 -Encoded Structural Protein Detected in Infected-Cell Complexes with ODV-EC27 and p78/83

Abstract

ABSTRACT orf101 is a late gene of Autographa californica nucleopolyhedrovirus (Ac M NPV). It encodes a protein of 42 kDa which is a component of the nucleocapsid of budded virus (BV) and occlusion-derived virus (ODV). To reflect this viral localization, the product of orf101 was named BV/ODV-C42 (C42). C42 is predominantly detected within the infected-cell nucleus: at 24 h postinfection (p.i.), it is coincident with the virogenic stroma, but by 72 h p.i., the stroma is minimally labeled while C42 is more uniformly located throughout the nucleus. Yeast two-hybrid screens indicate that C42 is capable of directly interacting with the viral proteins p78/83 ( 1629K ) and ODV-EC27 ( orf144 ). These interactions were confirmed using blue native gels and Western blot analyses. At 28 h p.i., C42 and p78/83 are detected in two complexes: one at approximately 180 kDa and a high-molecular-mass complex (500 to 600 kDa) which also contains EC27.