Molecular typing of Salmonella enterica serovar typhi

Author:

Navarro F1,Llovet T1,Echeita M A1,Coll P1,Aladueña A1,Usera M A1,Prats G1

Affiliation:

1. Servei de Microbiologia, Hospital de Sant Pau, Barcelona, Spain.

Abstract

The efficiencies of different tests for epidemiological markers--phage typing, ribotyping, IS200 typing, and pulsed-field gel electrophoresis (PFGE)--were evaluated for strains from sporadic cases of typhoid fever and a well-defined outbreak. Ribotyping and PFGE proved to be the most discriminating. Both detected two different patterns among outbreak-associated strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference19 articles.

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2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1991. Current protocols in molecular biology supplement 13 p. 2.4.1-2.4.5. Greene Publishing Associates and Wiley Interscience New York.

3. Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus;Bannerman T. L.;J. Clin. Microbiol.,1995

4. Comparative evaluation of molecular typing of strains from a national epidemic due to Salmonella brandenburg by rRNA gene and IS200 probes and pulsed-field gel electrophoresis;Baquar N.;J. Clin. Microbiol.,1994

5. Further consideration of the clonal nature of Salmonella typhi: evaluation of molecular and clinical characteristics of strains from Indonesia and Peru;Franco A.;J. Clin. Microbiol.,1992

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