Evaluation of Nanopore Sequencing as a Diagnostic Tool for the Rapid Identification of Mycoplasma bovis from Individual and Pooled Respiratory Tract Samples-Reference-Cited by-同舟云学术

Evaluation of Nanopore Sequencing as a Diagnostic Tool for the Rapid Identification of Mycoplasma bovis from Individual and Pooled Respiratory Tract Samples

Published:2021-11-18 Issue:12 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bokma Jade¹²^ORCID,Vereecke Nick³⁴^ORCID,Pas Mathilde L.¹,Chantillon Laurens¹,Vahl Marianne⁵,Weesendorp Eefke⁵,Deurenberg Ruud H.⁵,Nauwynck Hans³⁴,Haesebrouck Freddy²,Theuns Sebastiaan³⁴,Boyen Filip²,Pardon Bart¹

Affiliation:

1. Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

2. Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

3. Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

4. PathoSense BV, Lier, Belgium

5. Department of Diagnostics and Crisis Organization, Wageningen Bioveterinary Research, Lelystad, The Netherlands

Abstract

Rapid identification of Mycoplasma bovis infections in cattle is a key factor to guide antimicrobial therapy and biosecurity measures. Recently, Nanopore sequencing became an affordable diagnostic tool for both clinically relevant viruses and bacteria, but the diagnostic accuracy for M. bovis identification is undocumented.

Funder

Agentschap Innoveren en Ondernemen

FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01110-21

Reference50 articles.

1. Mycoplasma bovis Infections in Young Calves

2. Mycoplasma bovis in Respiratory Disease of Feedlot Cattle