Affiliation:
1. Department of Medical Microbiology, Academic Medical Centre, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
Abstract
ABSTRACT
Borrelia burgdorferi
sensu lato A14S was cultured from a skin biopsy specimen of a patient with erythema migrans in The Netherlands. This isolate had a unique DNA fingerprint pattern compared to 135 other
B. burgdorferi
sensu lato isolates. In this study, the isolate A14S was further characterized by protein analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reactivity with various monoclonal antibodies. In addition, the 16S rRNA,
ospA
, and
ospC
genes, as well as the 5S-23S rRNA intergenic spacer DNA, were amplified by PCR, cloned, and sequenced. SDS-PAGE protein profiles and phylogenetic analysis based on all of the analyzed genes confirmed that
B. burgdorferi
sensu lato A14S was phenotypically and genetically different from the three human pathogenic species
B. burgdorferi
sensu stricto,
Borrelia garinii
, and
Borrelia afzelii
, as well as from other
B. burgdorferi
sensu lato species. Our findings indicate that
Borrelia
genomic groups or isolates other than the three well-known human pathogenic species may also cause human Lyme borreliosis.
Publisher
American Society for Microbiology
Cited by
81 articles.
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