Effects of Aeration on the Synthesis of Poly(3-Hydroxybutyrate) from Glycerol and Glucose in Recombinant Escherichia coli-Reference-Cited by-同舟云学术

Effects of Aeration on the Synthesis of Poly(3-Hydroxybutyrate) from Glycerol and Glucose in Recombinant Escherichia coli

Published:2010-03-15 Issue:6 Volume:76 Page:2036-2040
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

de Almeida Alejandra¹,Giordano Andrea M.¹,Nikel Pablo I.¹²,Pettinari M. Julia¹

Affiliation:

1. Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires

2. Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Buenos Aires, Argentina

Abstract

ABSTRACT Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02706-09

Reference25 articles.

1. Production of Poly(3-Hydroxybutyrate) by Fed-Batch Culture of Recombinant Escherichia coli with a Highly Concentrated Whey Solution

2. Ashby, R. D., D. K. Y. Solaiman, and T. Foglia. 2005. Synthesis of short-/medium-chain-length poly(hydroxyalkanoate) blends by mixed culture fermentation of glycerol. Biomacromolecules6:2106-2112.

3. Borman, E. J., and M. Roth. 1999. The production of polyhydroxybutyrate by Methylobacterium rhodesianum and Ralstonia eutropha in media containing glycerol and casein hydrolysates. Biotechnol. Lett.21:1059-1063.

4. Cavalheiro, J. M. B. T., M. C. M. D. de Almeida, C. Grandfils, and M. M. R. da Fonseca. 2009. Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochem.44:509-515.

5. Dawes, E. A., and P. J. Senior. 1973. The role and regulation of energy reserve polymers in micro-organisms. Adv. Microb. Physiol.10:135-266.

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