Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments-Reference-Cited by-同舟云学术

Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic Sediments

Published:2009-06-15 Issue:12 Volume:75 Page:3972-3979
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Jogler Christian¹,Lin Wei²,Meyerdierks Anke³,Kube Michael⁴,Katzmann Emanuel¹,Flies Christine³,Pan Yongxin²,Amann Rudolf³,Reinhardt Richard⁴,Schüler Dirk¹

Affiliation:

1. LMU Department Biology I, Microbiology, Großharderner-Str. 2, 82152 Planegg-Martinsried, Germany

2. Biogeomagnetism Group, Paleomagnetism and Geochronology Laboratory, State Key Laboratory of Lithospheric Evolution, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China

3. Max Planck Institute for Marine Microbiology, Celsiusstr. 1, 28359 Bremen, Germany

4. Max Planck Institute for Molecular Genetics, Ihnestr. 73, 14195 Berlin, Germany

Abstract

ABSTRACT In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fosmid libraries were constructed and screened by end sequencing and hybridization analysis using heterologous magnetosome gene probes. A total of 14 fosmids were fully sequenced. We identified and characterized two fosmids, most likely originating from two different alphaproteobacterial strains of MTB that contain several putative operons with homology to the magnetosome island (MAI) of cultivated MTB. This is the first evidence that uncultivated MTB exhibit similar yet differing organizations of the MAI, which may account for the diversity in biomineralization and magnetotaxis observed in MTB from various environments.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02701-08

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