Affiliation:
1. Key Laboratory of Synthetic Biology, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
Abstract
ABSTRACT
Previous reports showed that
Streptomyces
linear plasmids usually contain one internal replication locus. Here, we identified two new replication loci on pFRL1, one (
rep1A-ncs1
) next to a telomere and another (
rep2A-ncs2
) ∼10 kb from it. The
rep1A-ncs1
locus was able to direct replication independently in both linear and circular modes, whereas
rep2A-ncs2
required an additional locus,
rlrA-rorA
, in order to direct propagation in linear mode. Rep1A protein bound to
ncs1 in vitro
. By quantitative reverse transcription-PCR and Northern hybridization, we showed that transcription of
rep1A
and
rep2A
varied during development and that each dominated at different time points. pFRL1-derived linear plasmids were inherited through spores more stably than circular plasmids and were more stable with pSLA2 telomeres than with pFRL1 telomeres in
Streptomyces lividans
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
7 articles.
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