Metagenomics-Guided Discovery of Potential Bacterial Metallothionein Genes from the Soil Microbiome That Confer Cu and/or Cd Resistance

Author:

Li Xiaofang1,Islam M. Mominul12,Chen Liang1,Wang Likun1,Zheng Xin1

Affiliation:

1. Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Centre for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China

2. University of Chinese Academy of Sciences, Beijing, China

Abstract

The metagenomics-guided procedure developed here bypasses the difficulties encountered in classic PCR-based approaches and led to the discovery of novel MT genes, which may be useful in developing bioremediation tools. The procedure used here expands our knowledge on the diversity of bacterial MTs in the environment and may also be applicable to identify other functional genes from eDNA.

Funder

National key research and development program of china

Hebei Natural Science Foundation for distinguished young scholars

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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