Affiliation:
1. Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455
Abstract
We have studied the effect of delta mutations in phage lambda on DNA synthesis as assayed by the accumulation of λ DNA in infected cells. We find that delta mutants appear to generate somewhat less DNA than λ
+
in a
rec
+
host, suggesting the wild-type delta gene may act in DNA replication. An additional clue to delta function arises if replication is measured in the gamma-negative situation where concatemer formation is abortive. In this situation, the wild-type delta gene has an “inhibitory” effect on replication. A similar inhibitory effect on replication due to delta is observed after infection of P
2
lysogens. We conclude from these studies that the delta gene may act with alpha, beta, and gamma genes, possibly in a process affecting DNA replication.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
4 articles.
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