Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene

Author:

Thylén C1

Affiliation:

1. Department of Microbiology, Faculty of Pharmacy, Uppsala University, Sweden.

Abstract

A plasmid expressing the cloned bacteriophage T4 gene 42 gave the same levels of complementation of gene 42 mutants in a polarity-suppressing rho mutant as in a rho+ host. A reading frame likely corresponding to gene 42 and putative promoter and terminator sequences were identified in the partial sequence of the cloned fragment.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference36 articles.

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3. A computer algorithm for testing potential procaryotic terminators;Brendel V.;Nucleic Acids Res.,1984

4. Brody E. D. Rabussay and D. H. Hall. 1983. Regulation of transcription of prereplicative genes p. 174-183. In C. K. Mathews E. M. Kutter G. Mosig and P. B. Berget (ed.) Bacteriophage T4. American Society for Microbiology Washington D.C.

5. A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro;Gram H.;Mol. Gen. Genet.,1984

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