Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria

Author:

Dix K1,Watanabe S M1,McArdle S1,Lee D I1,Randolph C1,Moncla B1,Schwartz D E1

Affiliation:

1. Molecular Biology Division, MicroProbe Corporation, Bothell, Washington 98021.

Abstract

Oligodeoxynucleotide probes were developed for identification of the periodontal bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius types I and II, B. forsythus, Eikenella corrodens, Fusobacterium nucleatum, Haemophilus aphrophilus, Streptococcus intermedius, and Wolinella recta. Probes were designed by sequencing the 16S rRNA for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. These probes were specific when tested against a panel of nucleic acids from closely related bacteria.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference25 articles.

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