Recognition of group B streptococci in dip-slide cultures of urine

Author:

Jokipii A M,Jokipii L

Abstract

One hundred strains of group B streptococci isolated from human infections were tested for growth on dip-slides available for the culture of urine. All grew on CLED agar, and none grew on MacConkey agar. The colonies were barely or not at all visible to the naked eye after overnight incubation (diameter, around 0.1 mm). The colony size increased eith prolonged incubation, but not if the inoculum density exceeded 10(6)/ml. Differences were found between lots of dip-slides. Poor growth on dip-slides may explain why group B streptococci have received little attention as pathogens of the urinary tract. The dip-slide screening personnel of one laboratory were informed of the experimental findings, and they started the practice of frequent subculture and prolonged incubation. The proportion of group B streptococci in significant bacteriuria increased from 0 to about 2% of positive cultures, whereas there was no conmitant increase of group B streptococci in dip-slides screened in several other laboratories serving as controls.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference11 articles.

1. Double-zone beta-hemolytic streptococci. Their cultural characteristics, serological grouping, occurrence and pathogenic significance;Brown J. H.;J. Bacteriol.,1939

2. Detection of bacteriuria by dip-slide. Routine use in a large general hospital;Ellner P. D.;Am. J. Clin. Pathol.,1975

3. Extrarespiratory streptococcal infections. Importance of the various serological groups;Feingold D. S.;N. Engl. J. Med.,1966

4. Dip-slide: an aid to quantitative urine culture in general practice;Guttmann D.;Br. Med. J.,1967

5. Presumptive identification and antibiotic susceptibility of group B streptococci;Jokipii A. M. M.;J. Clin. Pathol.,1976

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