Whole-Genome Sequencing Confirms the Coexistence of Different Colonizing Group B Streptococcus Isolates Underscored by CRISPR Typing-Reference-Cited by-同舟云学术

Whole-Genome Sequencing Confirms the Coexistence of Different Colonizing Group B Streptococcus Isolates Underscored by CRISPR Typing

Published:2020-01-30 Issue:5 Volume:9 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Beauruelle Clémence¹²³,Branger Maxime²,Cochard Thierry²,Pastuszka Adeline¹²³,Biet Franck²,Lanotte Philippe¹²³

Affiliation:

1. Université de Tours, INRAE, ISP, Tours, France

2. INRAE, Université de Tours, ISP, Nouzilly, France

3. CHRU de Tours, Service de Bactériologie-Virologie, Tours, France

Abstract

Streptococcus agalactiae is a major pathogen and is the leading cause of neonatal infections in industrialized countries. The diversity of strains isolated from two pregnant women was investigated. Here, we present the draft genome sequences of strains W8A2, W8A6, W10E2, and W10F3, obtained in order to ascertain their phylogenetic affiliation.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MRA.01359-19

Reference10 articles.

1. Maternal carriage of group B streptococci in developing countries

2. Group B Streptococcus Vaginal Carriage in Pregnant Women as Deciphered by Clustered Regularly Interspaced Short Palindromic Repeat Analysis

3. Andrews S. 2010. FastQC: a quality control tool for high throughput sequence data. http://www.bioinformatics.babraham.ac.uk/projects/fastqc.

4. Joshi NA Fass JN. 2011. Sickle: a sliding-window adaptive quality-based trimming tool for FastQ files (version 1.33). https://github.com/najoshi/sickle.

5. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing

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1. RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for screening multiple colonies;Journal of Microbiological Methods;2021-11

2. CRISPR Typing Increases the Discriminatory Power of Streptococcus agalactiae Typing Methods;Frontiers in Microbiology;2021-07-19

3. Application of the appropriate molecular biology-based method significantly increases the sensitivity of group B streptococcus detection results;Journal of Hospital Infection;2021-06