Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri : Purification of 1,3-Propanediol:NAD + Oxidoreductase-Reference-Cited by-同舟云学术

Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri : Purification of 1,3-Propanediol:NAD + Oxidoreductase

Published:1990-04 Issue:4 Volume:56 Page:943-948
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Talarico Todd L.¹,Axelsson Lars T.¹,Novotny James¹,Fiuzat Mitra¹,Dobrogosz Walter J.¹

Affiliation:

1. Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695, and Department of Microbiology, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden2

Abstract

Lactobacillus reuteri utilizes exogenously added glycerol as a hydrogen acceptor during carbohydrate fermentations, resulting in higher growth rates and cell yields than those obtained during growth on carbohydrates alone. Glycerol is first converted to 3-hydroxypropionaldehyde by a coenzyme B 12 -dependent glycerol dehydratase and then reduced to 1,3-propanediol by an NAD + -dependent oxidoreductase. The latter enzyme was purified and determined to have a molecular weight of 180,000; it is predicted to exist as a tetramer of identical 42,000-molecular-weight subunits.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.4.943-948.1990

Reference29 articles.

1. Production of a broad spectrum antimicrobial substance by Lactobacillus reuteri;Axelsson L. T.;Microb. Ecol. Health Dis.,1989

2. Characterization and DNA homology of Lactobacillus strains isolated from pig intestine;Axelsson L. T.;J. Appl. Bacteriol.,1987

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M.;Anal. Biochem.,1976

4. In vitro studies on reuterin synthesis by Lactobacillus reuteri;Chung T. C.;Microb. Ecol. Health Dis.,1989

5. Acrolein determination by means of tryptophane. A colorimetric micromethod;Circle S. J.;Ind. Eng. Chem. Anal. Ed.,1945

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