Utilization of the Methoxymalonyl-Acyl Carrier Protein Biosynthesis Locus for Cloning of the Tautomycin Biosynthetic Gene Cluster from Streptomyces spiroverticillatus-Reference-Cited by-同舟云学术

Utilization of the Methoxymalonyl-Acyl Carrier Protein Biosynthesis Locus for Cloning of the Tautomycin Biosynthetic Gene Cluster from Streptomyces spiroverticillatus

Published:2006-06 Issue:11 Volume:188 Page:4148-4152
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Li Wenli¹,Ju Jianhua¹,Osada Hiroyuki²,Shen Ben¹³⁴

Affiliation:

1. Division of Pharmaceutical Sciences

2. Antibiotics Laboratory, Discovery Research Institute, RIKEN, Wako 351-0198, Japan

3. University of Wisconsin National Cooperative Drug Discovery Group

4. Department of Chemistry, University of Wisconsin—Madison, Madison, Wisconsin 53705

Abstract

ABSTRACT Tautomycin (TTM), a potent protein phosphatase inhibitor, consists of a polyketide chain containing a spiroketal moiety and an acyl chain bearing a dialkylmaleic anhydride structure. PCR using degenerate primers was used to clone genes from Streptomyces spiroverticillatus for formation of the methoxymalonyl-acyl carrier protein. This locus was found to contain five genes ( ttmC , ttmA , ttmD , ttmB , and ttmE ), one of which was used as a probe to clone the 110-kb TTM biosynthetic gene cluster. The involvement of the ttmA gene in TTM biosynthesis was confirmed by gene inactivation and mutation complementation experiments.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00172-06

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