SODIUM AZIDE SELECTIVE MEDIUM FOR THE PRIMARY ISOLATION OF ANAEROBIC BACTERIA

Abstract

Forget , A. (University of Montreal, Montreal, Canada) and V. Fredette . Sodium azide selective medium for the primary isolation of anaerobic bacteria. J. Bacteriol. 83: 1217–1223. 1962.—A selective medium has been devised for the primary isolation of anaerobic bacteria from a mixture of both aerobes and anaerobes. The solid medium contains 0.05% NaN 3 , and the liquid medium has 0.2% NaN 3 in an identical base, namely, Trypticase soy broth. Addition of either 5% blood or 10% normal serum does not alter the selective action of sodium azide. The only defect which the medium exhibits is that it is unable to limit the growth of the facultative streptococci.