Mutation and Selection of Lactobacillus plantarum Strains That Do Not Produce Carbon Dioxide from Malate

Author:

Daeschel M. A.1,McFeeters R. F.1,Fleming H. P.1,Klaenhammer T. R.1,Sanozky R. B.1

Affiliation:

1. Food Fermentation Laboratory, Agricultural Research Service, U.S. Department of Agriculture, and North Carolina Agricultural Research Service, 2 Department of Food Science, North Carolina State University, Raleigh, North Carolina 27650

Abstract

A differential medium was developed to distinguish between malate-decarboxylating (MDC + ) and -non-decarboxylating (MDC ) strains of Lactobacillus plantarum. MDC strains produced a visible acid reaction in the medium, whereas MDC + strains did not. Use of the medium allowed for rapid screening and isolation of mutagenized cells that had lost the ability to produce CO 2 from malate.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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