Affiliation:
1. Pioneering Laboratory for Microbiological Chemistry, Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois
Abstract
Weimberg, Ralph
(Northern Regional Research Laboratory, Peoria, Ill.),
and William L. Orton
. Elution of exocellular enzymes from
Saccharomyces fragilis
and
Saccharomyces cerevisiae
. J. Bacteriol.
91:
1–13. 1966.—Invertase and acid phosphatase are repressible exocellular enzymes in
Saccharomyces fragilis
and
S. cerevisiae
. The conditions for eluting these enzymes from both organisms were compared. Either KCl or β-mercaptoethanol eluted the enzymes from
S. fragilis
, and the amounts eluted varied quantitatively according to the physiological age of the organism. In addition to eluting enzymatic activity from the cells, these reagents also caused a large increase in the amount of activity that remained associated with the cells of
S. fragilis
. Invertase and acid phosphatase were not removed from cells of
S. cerevisiae
by KCl or β-mercaptoethanol. These enzymes were separated from
S. cerevisiae
cells only when there was some degree of cell-wall digestion by snail gut fluid.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference22 articles.
1. Some observations on the form and location of invertase in the yeast cell;BURGER M., E.;Biochem. J.,1961
2. The effect of ,B-mercaptoethanol on release of invertase and formation of protoplasts of Saccharomyces fragilis;DAVIES R.;Biochem. J.,1964
3. Factors influencing the appearance of invertase in Saccharomyces cerevisiae;DODYK F.;Arch. Biochem. Biophys.,1964
4. Production of extracellular invertase by the yeast, Saccharomyces uvarum NRRL Y-972;ORSCHAK R. G.;Arch. Biochem. Biophys.,1958
5. Production of extracellular and total invertase by Candida utilis, Saccharomyces cerevisiae, and other yeasts;DWORSCHACK R. G.;Appl. Microbiol.,1961
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