Comparison of the Xpert Methicillin-Resistant Staphylococcus aureus (MRSA) Assay, BD GeneOhm MRSA Assay, and Culture for Detection of Nasal and Cutaneous Groin Colonization by MRSA

Author:

Kelley P. G.12,Grabsch E. A.1,Howden B. P.12,Gao W.1,Grayson M. L.1234

Affiliation:

1. Infectious Diseases

2. Microbiology Departments, Austin Health, Heidelberg, Victoria

3. Department of Epidemiology and Preventive Medicine, Monash University, Clayton, Victoria

4. Department of Medicine, University of Melbourne, Parkville, Victoria, Australia

Abstract

ABSTRACT Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and specificities (93.8% versus 92.7%, respectively). Combined PCR assays provide a rapid and more-complete assessment of colonization at a cost similar to that of single-site analysis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

1. Bannerman, T. L., and S. L. Peacock. 2007. Staphylococcus, Micrococcus, and other catalase-positive cocci, p. 390-411. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry, and M. A. Pfaller (ed.), Manual of clinical microbiology, 9th ed., vol. 1. ASM Press, Washington, DC.

2. BD GeneOhm MRSA assay product information. 2007

3. Concurrent Analysis of Nose and Groin Swab Specimens by the IDI-MRSA PCR Assay Is Comparable to Analysis by Individual-Specimen PCR and Routine Culture Assays for Detection of Colonization by Methicillin-Resistant Staphylococcus aureus

4. Xpert MRSA product information ref GXMRSA-100N-10. 2007

5. Performance standards for antimicrobial susceptibility testing. 2007

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