Cell envelope of Neisseria gonorrhoeae: phospholipase activity and its relationship to autolysis

Author:

Cacciapuoti A F,Wegener W S,Morse S A

Abstract

The relationship between conditions which permit or inhibit cell lysis and those which promote phospholipid hydrolysis in Neisseria gonorrhoeae was investigated. Suspension of exponential-phase gonococci in buffer in the absence of divalent cations resulted in autolysis but not in phosphlipid hydrolysis. The addition of Ca2+ or Mg2+ to the buffer inhibited autolysis and markedly stimulated the hydrolysis of phosphatidylethanolamine. Incubation of cells in buffer at pH 6 inhibited both autolysis and phospholipid hydrolysis.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference13 articles.

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5. Kates M. 1972. Techniques of lipidology: isolation analysis and identification of lipids p. 268-600. In T. S. Work and E. Work (ed.) Laboratory techniques in biochemistry and molecular biology vol. 3 North Holland Publishing Co. Amsterdam.

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