Author:
Turkington E O,Welker N E
Abstract
A thermostable lytic endopeptidase from Bacillus stearothermophilus 1503-4R was purified 14,500-fold, with a 34% recovery of lytic activity. The enzyme is a basic protein (pI, 9.7) with a molecular weight of 15,100 and is composed of approximately 129 amino acid residues.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference10 articles.
1. The spectrophotometric determination of tyrosine and tryptophan in protein;Goodwin T. W.;Biochem. J.,1946
2. Haschemeyer R. H. and A. E. U. Haschemeyer. 1973. Proteins. A guide to study by physical and chemical methods. John Wiley & Sons Inc. New York.
3. Determination of cystine as cysteic acid;Hirs C. H. W.;Methods Enzymol.,1967
4. Protein measurement with the Folin phenol reagent;Lowry 0.;J. Biol. Chem.,1951
5. Maurer H. R. 1971. Disc electrophoresis. Walter de Gruyter Inc. Hawthorne N.Y. 9