Comparison of an Automated Ribotyping System to Restriction Endonuclease Analysis and Pulsed-Field Gel Electrophoresis for Differentiating Vancomycin-Resistant Enterococcus faecium Isolates-Reference-Cited by-同舟云学术

Comparison of an Automated Ribotyping System to Restriction Endonuclease Analysis and Pulsed-Field Gel Electrophoresis for Differentiating Vancomycin-Resistant Enterococcus faecium Isolates

Published:2002-05 Issue:5 Volume:40 Page:1858-1861
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Price Connie S.¹,Huynh Holly²,Paule Suzanne³,Hollis Richard J.²,Noskin Gary A.⁴³,Pfaller Michael A.²,Peterson Lance R.¹⁴³

Affiliation:

1. Department of Pathology

2. Department of Pathology, University of Iowa, Iowa City, Iowa

3. Northwestern Memorial Hospital, Chicago, Illinois

4. Department of Medicine, Northwestern University

Abstract

ABSTRACT The RiboPrinter Microbial Characterization System was compared with pulsed-field gel electrophoresis (PFGE), restriction endonuclease analysis (REA), and epidemiological data for typing 45 vancomycin-resistant Enterococcus faecium (VRE) isolates. In 21 clinically related isolates, 90 to 100% were similar by PFGE and REA, but only 57% were similar by the RiboPrinter. In another eight clinically related isolates, three isolates similar by PFGE and REA were all unique by the RiboPrinter. In contrast, in 16 clinically unrelated isolates, the predominant RiboPrinter ribotype represented 50% of the strains, while the largest PFGE and REA clones represented less than 19% of the strains. These data suggest that the RiboPrinter is not reliable for VRE investigation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.5.1858-1861.2002

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