Analysis of Immunoglobulin A Antibodies to Helicobacter pylori in Serum and Gastric Juice in Relation to Mucosal Inflammation

Author:

Hayashi Shunji1,Sugiyama Toshiro2,Yokota Kenji3,Isogai Hiroshi4,Isogai Emiko5,Oguma Keiji3,Asaka Masahiro2,Fujii Nobuhiro6,Hirai Yoshikazu1

Affiliation:

1. Department of Microbiology, Jichi Medical School, Tochigi-ken 329-0498,1

2. Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo 060-8638,2

3. Department of Bacteriology, Okayama University Medical School, Okayama 700-8558,3

4. Animal Experimentation Center4 and

5. Department of Hygiene, Health Sciences University of Hokkaido, Hokkaido 061-0293,5 Japan

6. Department of Microbiology,6 Sapporo Medical University School of Medicine, Sapporo 060-8556, and

Abstract

ABSTRACT Helicobacter pylori is a major etiologic agent in gastroduodenal disorders. In this study, immunoglobulin A (IgA) antibodies to H. pylori antigens were evaluated in serum and gastric juice specimens obtained from patients with gastritis or peptic ulcers by utilizing antibody capture enzyme-linked immunosorbent assays (ACELISAs). Urease α subunit (UA), urease β subunit (UB), the 66-kDa heat shock protein (HSP), and the 25-kDa protein (25K) were used as antigens for the ACELISAs. The antibody titers of the ACELISAs reflect the ratio of H. pylori -specific IgA to total IgA. The ratio is stable, although the antibody concentration fluctuates in gastric juice. By using ACELISAs it was possible to evaluate quantitatively not only serum IgA antibodies but also gastric juice secretory IgA (S-IgA) antibodies. In both serum IgA and gastric juice S-IgA ACELISAs, the titers of antibody to HSP and 25K were remarkably correlated with the histologic grade of gastritis, whereas those to UA and UB were not strongly correlated with histologic grade. Thus, it is useful for estimating the histologic grade of gastritis to quantify serum IgA and gastric juice S-IgA antibodies to HSP and 25K.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

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