Affiliation:
1. Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan
Abstract
ABSTRACT
To evaluate the denitrification abilities of many
Bradyrhizobium
field isolates, we developed a new
15
N-labeled N
2
detection methodology, which is free from interference from atmospheric N
2
contamination.
30
N
2
(
15
N
15
N) and
29
N
2
(
15
N
14
N) were detected as an apparent peak by a gas chromatograph equipped with a thermal conductivity detector with N
2
gas having natural abundance of
15
N (0.366 atom%) as a carrier gas. The detection limit was 0.04%
30
N
2
, and the linearity extended at least to 40%
30
N
2
. When
Bradyrhizobium japonicum
USDA110 was grown in cultures anaerobically with
15
NO
3
−
, denitrification product (
30
N
2
) was detected stoichiometrically. A total of 65 isolates of soybean bradyrhizobia from two field sites in Japan were assayed by this method. The denitrification abilities were partly correlated with filed sites,
Bradyrhizobium
species, and the
hup
genotype.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
25 articles.
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