Affiliation:
1. Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France
Abstract
ABSTRACT
We identified a polyketide synthase (PKS) gene,
pksN
, from a strain of
Nectria haematococca
by complementing a mutant unable to synthesize a red perithecial pigment.
pksN
encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: β-ketoacyl synthase, acyltransferase, duplicated acyl carrier proteins, and thioesterase. The
pksN
product groups with the
Aspergillus nidulans
WA-type PKSs involved in conidial pigmentation and melanin, bikaverin, and aflatoxin biosynthetic pathways. Inactivation of
pksN
did not cause any visible change in fungal growth, asexual sporulation, or ascospore formation, suggesting that it is involved in a specific developmental function. We propose that
pksN
encodes a novel PKS required for the perithecial red pigment biosynthesis.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference47 articles.
1. Basic local alignment search tool
2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. A. Smith J. G. Seidman and K. Struhl. 1987. Current protocols in molecular biology. John Wiley and Sons New York N.Y.
3. Babai-Ahary, A., M. J. Daboussi-Bareyre, and D. Parisot. 1982. Isolation and genetic analysis of self-sterility and perithecial pigmentation mutants in the homothallic isolate of Nectria haematococca.Can. J. Bot.60:79-84.
4. Beck, J., S. Ripka, A. Siegner, E. Schiltz, and E. Schweizer. 1990. The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases. Eur. J. Biochem.192:487-498.
5. Bingle, L. E., T. J. Simpson, and C. M. Lazarus. 1999. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Fungal Genet. Biol.26:209-223.
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