Author:
Atkins E,Francis L,Bernheim H A
Abstract
The present studies were designed to investigate the role of monocytes in the pathogenesis of fever in delayed hypersensitivity. Adherent rabbit blood monocytes (from both normal and sensitized donors) were separated on Ficoll-Hypaque gradients and incubated with antigen (Ag; ovalbumin) and sensitized draining-lymph-node lymphocytes (or their supernatants) from rabbits with delayed hypersensitivity, and release of endogenous pyrogen was assayed. Results indicated that monocytes are activated to produce endogenous pyrogen by Ag and suspensions of draining-lymph-node cells or by an agent (lymphokine) in the supernatants of sensitized lymphocytes preincubated with Ag. The release of lymphokine was Ag specific and was correlated with the skin test reactivity of the donor rabbits to the sensitizing Ag. No evidence was found that Ag-antibody complexes or (in the case of sensitized monocytes) cytophilic antibodies play a role in the activity of this lymphokine which appears to act selectively on monocytes rather than on granulocytes.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference16 articles.
1. Studies on the mechanism of fever accompanying delayed hypersensitivity. The role of the sensitized Iymphocyte;Atkins E.;J. Exp. Med.,1972
2. Pathogenesis of fever in delayed hypersensitivity: factors influencing release of pyrogen-inducing Iymphokines;Atkins E.;Infect. Immun.,1978
3. Studies on the mechanism of endogenous pyrogen production. III. Human blood monocytes;Bodel P.;J. Exp. Med.,1974
4. Studies on steroid fever. I. Production of leukocyte pyrogen in vitro by etiocholanolone;Bodel P.;J. Clin. Invest.,1968
5. Studies on the pathogenesis of fever. XI. Quantitative features of the febrile response to leucocytic pyrogen;Bornstein D. L.;J. Exp. Med.,1963
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献