Critical Domains in the Specific Binding of Radiolabeled Vip3Af Insecticidal Protein to Brush Border Membrane Vesicles from Spodoptera spp. and Cultured Insect Cells

Author:

Quan Yudong1,Lázaro-Berenguer Maria1ORCID,Hernández-Martínez Patricia1ORCID,Ferré Juan1ORCID

Affiliation:

1. Instituto Universitario de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, Burjassot, Spain

Abstract

This study shows that Vip3Af binding sites are fully shared with Vip3Aa, only partially shared with Vip3Ca, and not shared with Cry1Ac and Cry1F in two Spodoptera spp. Truncated versions of Vip3Af revealed that only domains I to III were necessary for the specific binding, most likely because they can form the functional tetrameric oligomer and because domain III is supposed to contain the binding epitopes.

Funder

GVA | Conselleria de Cultura, Educación y Ciencia, Generalitat Valenciana

Ministerio de Ciencia, Innovación y Universidades

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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