Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay-Reference-Cited by-同舟云学术

Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay

Published:2011-10 Issue:10 Volume:49 Page:3681-3682
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Maass Viola¹,Kern Jan Marco¹,Poeckl Matthias¹,Maass Matthias¹

Affiliation:

1. Institute of Medical Microbiology, Hygiene and Infectious Diseases, PMU University Hospital Salzburg, Muellner Hauptstr. 48, 5020 Salzburg, Austria

Abstract

ABSTRACT Mycoplasma contamination is a frequent problem in chlamydial cell culture. After obtaining contradictory contamination results, we compared three commercial PCR kits for mycoplasma detection. One kit signaled contamination in mycoplasma-free Chlamydia pneumoniae cultures. Sequencing of cloned PCR products revealed primer homology with the chlamydial genome as the basis of this false-positive result.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01092-11

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