Author:
Caballero-Mellado J,Fuentes-Ramirez L E,Reis V M,Martinez-Romero E
Abstract
A total of 55 isolates of Acetobacter diazotrophicus recovered from diverse sucrose-rich host plants and from mealybugs associated with sugarcane plants were characterized by the electrophoretic mobilities of 12 metabolic enzymes. We identified seven different electrophoretic types (ETs), six of which are closely related within a genetic distance of 0.195 and exhibit high DNA-DNA homology. The seventh ET was largely divergent, separated at a genetic distance of 0.53, and had only 54% DNA homology to the reference strain. Strains corresponding to ET 7 could represent a distinct nitrogen-fixing species of the genus Acetobacter. More genetic diversity was found in isolates from Brazil than in those from Mexico, probably due to the very different crop nitrogen fertilization levels used.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference52 articles.
1. Acetic acid bacterial biota of the pink sugarcane mealybug, Saccharococcus sacchari, and its environs;Ashbolt N. J.;Appl. Environ. Microbiol.,1990
2. Ausubel F. M. R. Rent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1987. Current protocols in molecular biology. John Wiley and Sons Inc. New York.
3. Identification and ecology of Herbaspirillum seropedicae and the closely related Pseudomonas rubrisubalbicans;Baldani V. L. D.;Symbiosis,1992
4. Toward a population genetic analysis of Salmonella: genetic diversity and relationship among strains of serotypes S. choleraesuis, S. derby, S. dublin, S. enteritidis, S. heidelberg, S. infantis, S. newport, and S. typhimurium;Beltran P.;Proc. Natl. Acad. Sci. USA,1988
5. Biological nitrogen fixation associated with sugar cane;Boddey R. M.;Plant Soil,1991
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