Affiliation:
1. Instituto de Investigaciones Marinas, CSIC, 36208 Vigo, Spain
Abstract
ABSTRACT
In the present work, the effects of several lactic acid bacteria on the immune response of turbot (
Scophthalmus maximus
) macrophages have been studied both in vitro and in vivo. Out of six lactic acid bacterial strains tested, only heat-killed
Lactococcus lactis
significantly increased the turbot head kidney macrophage chemiluminescent (CL) response after 24 h of incubation. Nitric oxide (NO) was also significantly enhanced by this bacterium after 72 h of incubation with either viable (10
3
and 10
6
cells/ml) or heat-killed (10
6
cells/ml) bacteria. Viable
Leuconostoc mesenteroides
(10
6
cells/ml) was also capable of significantly increasing NO production. Since
L. lactis
proved to be the strain with more effects on the host immune function, further in vivo and in vitro experiments were conducted with this bacterium. The in vitro capacity of
L. lactis
to adhere to turbot intestinal mucus was positively confirmed. When orally administered,
L. lactis
significantly increased the macrophage CL response and the serum NO concentration after 7 days of daily administration. The antibacterial effect of the extracellular products from the six LAB strains against the fish-pathogenic bacterium
Vibrio anguillarum
was also demonstrated in vitro.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
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