Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and Persistence In Vivo-Reference-Cited by-同舟云学术

Autoinducer 2 (AI-2) Production by Nontypeable Haemophilus influenzae 86-028NP Promotes Expression of a Predicted Glycosyltransferase That Is a Determinant of Biofilm Maturation, Prevention of Dispersal, and Persistence In Vivo

Published:2018-12 Issue:12 Volume:86 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Pang Bing¹,Armbruster Chelsie E.¹^ORCID,Foster Gayle¹,Learman Brian S.¹,Gandhi Uma¹,Swords W. Edward¹²³

Affiliation:

1. Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA

2. Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA

3. Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA

Abstract

Nontypeable Haemophilus influenzae (NT Hi ) is an extremely common human pathobiont that persists on the airway mucosal surface within biofilm communities, and our previous work has shown that NT Hi biofilm maturation is coordinated by the production and uptake of autoinducer 2 (AI-2) quorum signals. To directly test roles for AI-2 in maturation and maintenance of NT Hi biofilms, we generated an NT Hi 86-028NP mutant in which luxS transcription was under the control of the xylA promoter (NT Hi 86-028NP luxS xylA :: luxS ), rendering AI-2 production inducible by xylose.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.00506-18

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