Control of Ferredoxin and Gal/GalNAc Lectin Gene Expression in Entamoeba histolytica by a cis -Acting DNA Sequence-Reference-Cited by-同舟云学术

Control of Ferredoxin and Gal/GalNAc Lectin Gene Expression in Entamoeba histolytica by a cis -Acting DNA Sequence

Published:1998-05 Issue:5 Volume:66 Page:2383-2386
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Gilchrist Carol A.¹,Mann Barbara J.¹²,Petri William A.¹²³

Affiliation:

1. Departments of Internal Medicine,1

2. Microbiology,2 and

3. Pathology,3 University of Virginia, Charlottesville, Virginia 22908

Abstract

ABSTRACT The ferredoxin ( fdx ) and lectin ( hgl 5) promoters of Entamoeba histolytica contain the DNA sequence motif TATTCTATT (URE3). Previously we showed that mutation of the URE3 motif in the hgl 5 lectin promoter results in an increase in promoter reporter activity. Mutation of this motif in the fdx promoter led to a 40-to-50% decrease in fdx promoter activity as measured by reporter gene activity and abundance of mRNA. E. histolytica nuclear proteins exhibited sequence-specific binding to the URE3 motif in electrophoretic mobility shift assays. These results support the regulation of both ferredoxin and lectin promoters by a nuclear protein(s) which recognizes the URE3 motif.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.5.2383-2386.1998

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