Construction and Characterization of an Isogenic slt-ii Deletion Mutant of Enterohemorrhagic Escherichia coli-Reference-Cited by-同舟云学术

Construction and Characterization of an Isogenic slt-ii Deletion Mutant of Enterohemorrhagic Escherichia coli

Published:1998-05 Issue:5 Volume:66 Page:2337-2341
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Gunzer Florian¹,Bohn Ursula¹,Fuchs Sibylle²,Mühldorfer Inge²,Hacker Jörg²,Tzipori Saul¹,Donohue-Rolfe Arthur¹

Affiliation:

1. Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536,1 and

2. Institut für Molekulare Infektionsbiologie der Universität Würzburg, 97070 Würzburg, Germany2

Abstract

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) produces Shiga-like toxins (SLT), potent protein synthesis inhibitors. To further dissect the role of SLT-II in the course of disease, we have constructed E. coli TUV86-2, an isogenic SLT-II-negative mutant of EHEC strain 86-24. The slt-ii gene was inactivated by suicide vector mutagenesis. We also isolated derivatives of strain 86-24 that were cured of the phage carrying the toxin genes.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.5.2337-2341.1998

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