Affiliation:
1. National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014
Abstract
Infection of nonlysogenic
Escherichia coli
CR34(S) (Thy
−
) with bacteriophage λ C
I
857 resulted in the formation of twisted circular double-stranded phage deoxyribonucleic acid (DNA; species I). When such infected bacteria were incubated in the absence of thymine, there was a significant decrease in the amount of species I DNA after 60 min of incubation. A similar loss of species I λ DNA during incubation in a thymine-deficient medium was also observed after infection of the endonuclease I-deficient strain,
E. coli
1100(S) (Thy
−
). This destruction of twisted, circular λ DNA in thymine-deprived cells did not occur in the presence of chloramphenicol nor in lysogenic
E. coli
CR34 carrying a noninducible λ prophage. It is therefore concluded that the endonuclease which attacks this circular configuration of λ DNA is newly synthesized after infection and is directed by the phage chromosome.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
17 articles.
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