Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis

Author:

Shatkin A. J.1,Sipe J. D.1,Loh P.1

Affiliation:

1. Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Double-stranded ribonucleic acid ( RNA ) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 × 10 6 daltons, respectively, or a total of 15 × 10 6 daltons.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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