Author:
Arnold M T,Grappel S F,Lerro A V,Blank F
Abstract
Two highly purified peptido polysaccharide antigens have been isolated from surface-grown cultures of Trichophyton mentagrophytes var. granulosum. Trichloroacetic acid extraction and ethanol precipitation yielded a mixture containing high-molecular-weight components which were first separated on Sephadex G-200. Subsequent fractionation by ion-exchange chromatography on DE-52-cellulose (borate form) yielded the two peptido polysaccharides. Both of the peptido polysaccharides reacted with rabbit antiserum to T. mentagrophytes var. granulosum. The two peptido polysaccharides contain 73.2% hexoses (mannose-galactose-glucose, 7.5:0.7:1), 8.6% amino acids and 1.8% amino sugars and 77.4% hexoses (mannose-galactose-glucose, 9:0.3:1), 6.2% amino acids, and 0.4% amino sugars, respectively. Each contains 16 different amino acids, threonine, proline, and serine predominating.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference24 articles.
1. Galactomannan peptides of Trichophyton mentagrophytes;Barker S. A.;Carbohydr. Res.,1967
2. The isolation of trichophytin glycopeptide and its structure in relation to the immediate and delayed reactions;Barker S. A.;Immunology,1962
3. Immunological relationships between glycopeptides of Microsporum canis, Trichophyton rubrum, Trichophyton mentagrophytes, and other fungi;Basarab O.;Sabouraudia,1968
4. The water-soluble polysaccharides of dermatophytes. V. Galactomannans II from Trichophyton granulosum, Trichophyton interdigitale, Microsporum quinckeanum, Trichophyton rubrum, and Trichophyton schonleinii;Bishop C. T.;Can. J. Chem.,1966
5. The water-soluble polysaccharides of dermatophytes. IV. Galactomannans I from Trichophyton granulosum, Trichophyton interdigitale, Microsporum quinckeanum, Trichophyton rubrum, and Trichophyton schonleinii;Bishop C. T.;Can. J. Chem.,1965
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