Molecular Characterization of Multidrug Resistance in Streptococcus mitis

Author:

Poutanen Susan M.12,de Azavedo Joyce13,Willey Barbara M.1,Low Donald E.123,MacDonald Kelly S.12

Affiliation:

1. Department of Microbiology, Mount Sinai and Princess Margaret Hospitals,1 and

2. Department of Medicine2 and

3. Department of Laboratory Medicine and Pathobiology,3 University of Toronto, Toronto, Ontario, Canada

Abstract

ABSTRACT Antimicrobial resistance was characterized for 14 strains of Streptococcus mitis . Hin fI restriction fragment length mapping of gyrA PCR amplicons from three ciprofloxacin-resistant isolates correlated with mutations associated with such resistance in other organisms. By using PCR, seven erythromycin-resistant strains were found to possess either the mef or ermB gene. Hybridization revealed tet (M) in seven tetracycline-resistant isolates.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference17 articles.

1. Identification of chromosomal antibiotic resistance genes in Streptococcus anginosus ("S. milleri")

2. Classification and identification of the viridans streptococci

3. Dantley K. A. Burdett V. Tet (M)-mediated tetracycline resistance: requirement for tet (M)-ribosome association abstr. C-112 Abstracts of the 37th Interscience Conference on Antimicrobial Agents and Chemotherapy. 1997 65 American Society for Microbiology Washington D.C

4. Emergence of high rates of antimicrobial resistance among viridans group streptococci in the United States

5. Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococci pneumoniae.;Dowson C. G.;Proc. Natl. Acad. Sci. USA,1990

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