Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)-Reference-Cited by-同舟云学术

Development of a Duplex Real-Time PCR Assay for the Differentiation of Blastomyces dermatitidis and Blastomyces gilchristii and a Retrospective Analysis of Culture and Primary Specimens from Blastomycosis Cases from New York (2005 to 2019)

Published:2021-02-18 Issue:3 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kaplan Mitchell¹,Zhu YanChun¹,Kus Julianne V.²³^ORCID,McTaggart Lisa²,Chaturvedi Vishnu¹⁴^ORCID,Chaturvedi Sudha¹⁴^ORCID

Affiliation:

1. Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA

2. Public Health Laboratory, Public Health Ontario, Toronto, Ontario, Canada

3. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

4. Department of Biomedical Sciences, University at Albany, Albany, New York, USA

Abstract

Blastomycosis due to Blastomyces dermatitidis and Blastomyces gilchristii is a significant cause of respiratory mycoses in North America with occasional reported outbreaks. We developed a highly sensitive, specific, and reproducible TaqMan duplex real-time PCR assay for the differentiation of B. dermatitidis and B. gilchristii .

Funder

National Science Foundation

HHS | Centers for Disease Control and Prevention

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02078-20

Reference29 articles.

1. A CASE OF PSEUDO-LUPUS VULGARIS CAUSED BY A BLASTOMYCES

2. Clinical and Laboratory Update on Blastomycosis