Evaluation of Low-Colony-Number Counts of Mycobacterium tuberculosis on Solid Media as a Microbiological Marker of Cross-Contamination-Reference-Cited by-同舟云学术

Evaluation of Low-Colony-Number Counts of Mycobacterium tuberculosis on Solid Media as a Microbiological Marker of Cross-Contamination

Published:2009-06 Issue:6 Volume:47 Page:1950-1952
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ribeiro F. K. C.¹,Lemos E. M.¹,Hadad D. J.¹,Leão S. C.²,Viana-Niero C.²,Dietze R.¹,Johnson J. L.³,Eisenach K. D.⁴,Palaci M.¹

Affiliation:

1. Núcleo de Doenças Infecciosas, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil

2. Departamento de Microbiologia, Imunologia, e Parasitologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil

3. Tuberculosis Research Unit, Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Case Medical Center, Cleveland, Ohio

4. Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas

Abstract

ABSTRACT Low-colony-number counts on solid media are considered characteristic of cross-contamination, although they are normally observed in true-positive cultures from some groups of patients. The aim of this study was to evaluate low-yield growth cultures as a microbiological marker for cross-contamination. We evaluated 106 cultures with <15 colonies from 94 patients, and the proportions of false-positive cultures were 0.9% per sample and 1.1% per patient, which indicates that low-yield growth is not a reliable marker of cross-contamination.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00626-09

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