Author:
Clancy S,Mann C,Davis R W,Calos M P
Abstract
Saccharomyces cerevisiae was transformed with DNA by the lithium acetate method. Mutation of nonselected markers on the transforming vector was observed at a frequency several orders of magnitude higher than spontaneous mutation frequencies. These mutations were shown to be deletions. Linearization of the vector before transformation stimulated deletion formation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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