Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci-Reference-Cited by-同舟云学术

Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci

Published:2008-03 Issue:3 Volume:46 Page:1118-1122
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zhang Kunyan¹²³⁴⁵,McClure Jo-Ann¹,Elsayed Sameer²³⁵,Louie Thomas³⁴⁵,Conly John M.¹²³⁴⁵

Affiliation:

1. Centre for Antimicrobial Resistance, Calgary Health Region, Calgary, Alberta, Calgary Laboratory Services, Calgary, Alberta, and University of Calgary, Calgary, Alberta, Canada

2. Departments of Pathology and Laboratory Medicine

3. Microbiology and Infectious Diseases

4. Medicine

5. Institute of Infection, Immunity and Inflammation, University of Calgary, Calgary, Alberta, Canada

Abstract

ABSTRACT We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01309-07

Reference15 articles.

1. CDC. 2003. Outbreaks of community-associated methicillin-resistant Staphylococcus aureus skin infections—Los Angeles County, California, 2002-2003. MMWR Morb. Mortal. Wkly. Rep.52:88.

2. Multilocus Sequence Typing for Characterization of Methicillin-Resistant and Methicillin-Susceptible Clones of Staphylococcus aureus

3. eBURST: Inferring Patterns of Evolutionary Descent among Clusters of Related Bacterial Genotypes from Multilocus Sequence Typing Data

4. Severe Community-Onset Pneumonia in Healthy Adults Caused by Methicillin-Resistant Staphylococcus aureus Carrying the Panton-Valentine Leukocidin Genes

5. Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management

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