Interlaboratory Comparison of Intact-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Results for Identification and Differentiation of Brucella spp-Reference-Cited by-同舟云学术

Interlaboratory Comparison of Intact-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Results for Identification and Differentiation of Brucella spp

Published:2013-09 Issue:9 Volume:51 Page:3123-3126
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Karger Axel¹,Melzer Falk²,Timke Markus³,Bettin Barbara¹,Kostrzewa Markus³,Nöckler Karsten⁴,Hohmann Angelika¹⁵,Tomaso Herbert²,Neubauer Heinrich²,Al Dahouk Sascha⁴⁶

Affiliation:

1. Friedrich-Loeffler-Institut, Institute of Molecular Biology, Greifswald-Insel Riems, Germany

2. Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, Germany

3. Bruker Daltonik GmbH, Bremen, Germany

4. Federal Institute for Risk Assessment, Berlin, Germany

5. Friedrich-Loeffler-Institut, Department of Experimental Animal Facilities and Biorisk Management, Greifswald-Insel Riems, Germany

6. RWTH Aachen University, Department of Internal Medicine III, Aachen, Germany

Abstract

ABSTRACT Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patterns allowed accurate identification to the genus level. However, statistical models based on peak intensities were needed for definite species differentiation. Interlaboratory comparison confirmed the reproducibility of the results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01720-13

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