Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product-Reference-Cited by-同舟云学术

Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product

Published:1992-02 Issue:3 Volume:174 Page:1050-1052
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Blanche F¹,Famechon A¹,Thibaut D¹,Debussche L¹,Cameron B¹,Crouzet J¹

Affiliation:

1. Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.

Abstract

A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.174.3.1050-1052.1992

Reference14 articles.

1. Purification of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Pseudomonas denitrificans;Blanche F.;J. Bacteriol.,1989

2. Precorrin-6x reductase from Pseudomonas denitrificans: purification and characterization of the enzyme and identification of the structural gene;Blanche F.;J. Bacteriol.,1992

3. Hydrogenobyrinic acid: isolation, biosynthesis, and function;Blanche F.;Angew. Chem. Int. Ed. EngI.,1990

4. Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid;Crouzet J.;J. Bacteriol.,1990

5. Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase;Crouzet J.;J. Bacteriol.,1990

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