Ultrasensitive Human Immunodeficiency Virus Type 1 p24 Antigen Assay Modified for Use on Dried Whole-Blood Spots as a Reliable, Affordable Test for Infant Diagnosis

Author:

Patton Janet C.1234,Sherman Gayle G.1234,Coovadia Ashraf H.1234,Stevens Wendy S.1234,Meyers Tammy M.1234

Affiliation:

1. Wits Paediatric HIV Unit, Wits Health Consortium

2. Departments of Molecular Medicine and Hematology

3. Paediatrics, University of the Witwatersrand, Johannesburg, South Africa

4. National Health Laboratory Service, South Africa

Abstract

ABSTRACT The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

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