Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin-Reference-Cited by-同舟云学术

Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin

Published:1984-03 Issue:3 Volume:19 Page:394-398
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Surujballi O P,Fackrell H B

Abstract

A sandwich enzyme-linked immunosorbent assay was developed for measuring Staphylococcus aureus alpha-toxin. This assay was 500 to 1,000 times more sensitive than the commonly used hemolytic titration assay and was less variable. The binding of alpha-toxin to the adsorbed antibody was most effective after an overnight incubation at 27 degrees C. The toxin was detectable even at a log2 17 dilution of an S. aureus culture supernatant.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.19.3.394-398.1984

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