Use of Single-Enzyme Amplified Fragment Length Polymorphism for Typing Pasteurella multocida subsp. multocida Isolates from Pigs

Author:

Moreno A. M.1,Baccaro M. R.1,Ferreira A. J. P.1,Pestana de Castro A. F.2

Affiliation:

1. Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo

2. Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-000 São Paulo, Sao Paulo, Brazil

Abstract

ABSTRACT Single-enzyme amplified fragment length polymorphism (SE-AFLP) analyses were used to differentiate 97 isolates of porcine Pasteurella multocida subsp. multocida . The strains, isolated from animals with pneumonia, rhinitis, and septicemia, were classified as capsular types A, D, and F. SE-AFLP showed a discriminatory index of 0.87 and identified 18 different profiles.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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2. Rapid and simple method for purification of nucleic acids

3. Borowski S. M. 2001. Tese de Doutorado. Universidade Federal do Rio Grande do Sul. Porto Alegre Brazil.

4. Bowles, R., J. L. Pahoff, B. N. Smith, and P. J. Blackal. 2000. Ribotype diversity of porcine Pasteurella multocida from Australia. Aust. Vet. J.78:630-635.

5. Brito, J. R. F., I. A. Piffer, I. Wentz, and M. A. V. P. Brito. 1993. Capsular types and toxin production by strains of Pasteurella multocida isolated from pigs in southern Brazil. Rev. Microbiol.24:94-97.

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